Journal: Biochemistry and biophysics reports

Article Title: Oxymatrine blocks the NLRP3 inflammasome pathway, partly downregulating the inflammatory responses of M1 macrophages differentiated from THP-1 monocytes.

doi: 10.1016/j.bbrep.2023.101482

Figure Lengend Snippet: Fig. 2. Morphological images of cells in different states and identification of M0 and M1 macrophages. (A) Morphology of THP-1 cells, M0 macrophages, and M1 macrophages. Scale bar: 200 μm. THP-1 monocytes were round, translucent, and suspended; M0 macrophages were clustered and adherent; M1 macrophages were adherent and can extend pseudopodias. (B) Immunofluorescence staining on M0 cells. Scale bar: 50 μm. Colocalization of CD68 (red) and DAPI (blue). (C) Immunofluorescence staining of M1 cells. Scale bar: 50 μm. Colocalization of CD86 (red) and DAPI (blue). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: For immunofluorescence staining, the cells were fixed with 4% paraformaldehyde (PFA) (Biosharp, Anhui, China) and incubated with diluted rabbit primary antibody against CD68 (1:300) (Boster, Wuhan, China) overnight at 4 ◦C.

Techniques: Immunofluorescence, Staining